製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P23560
    • 生物種Human
    • 配列MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
    • 分子量27 kDa
    • 領域129 to 247
    • 配列の追加情報aa 129 to 247 refers to the full length mature form. This product does not contain signal peptide or propeptide.

特性

Our Abpromise guarantee covers the use of ab9794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.

    The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions.

  • アプリケーション

    Celluar Activation

    SDS-PAGE

    Western blot

    Other

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 精製度> 98 % SDS-PAGE.
    Also greater than 98% pure by HPLC analyses.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

関連情報

  • 別名
    • Abrineurin
    • ANON2
    • BDNF
    • BDNF_HUMAN
    • Brain Derived Neurotrophic Factor
    • Brain-derived neurotrophic factor
    • BULN2
    • MGC34632
    • Neurotrophin
    see all
  • 機能During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • 組織特異性Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • 関連疾患Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • 配列類似性Belongs to the NGF-beta family.
  • 翻訳後修飾The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human BDNF protein (ab9794) 使用論文

This product has been referenced in:
  • Wessels JM  et al. The brain-uterus connection: brain derived neurotrophic factor (BDNF) and its receptor (Ntrk2) are conserved in the mammalian uterus. PLoS One 9:e94036 (2014). Read more (PubMed: 24714156) »
  • Chavez-Valdez R  et al. Sexual dimorphism in BDNF signaling after neonatal hypoxia-ischemia and treatment with necrostatin-1. Neuroscience 260:106-19 (2014). Read more (PubMed: 24361177) »

See all 5 Publications for this product

Product Wall


I heard back from both labs that produce the BDNF and pro-BDNF proteins, and both said that the reconstitution instructions should be followed directly.
BDNF ab9794: reconstitute in water
pro BDNF ab138787: reconstitute in PBS

Thank you for contacting us.
The lab let me know the following:
The protein is lyophilized with no additives, which means that it is HPLC purified. Therefore, the only buffer that would be present at lyophilization would be water and 0.1% TFA ...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Vorweg: Den Rabattcode können Sie ab sofort nutzen. Da der vorige unseren Unterlagen zufolge gerade abgelaufen ist, habe ich Ihnen nochmal einen neuen ausgestel...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit unserem BDNF-Antikörper und Proteinhaben.

Bezüglich des Gutscheincodes kann ich Ihnen mitteilen, dass ich die Ausnahme machen konnte, ...

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Thank you for your phone call. I am sending you the testing discount codes for the 2 proteins for trying them in a blocking experiment - see below.

Please send me your WB data so that I cantake a look at your results.
DISCOUNT CODE: xxx ...

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Thank you for contacting us. The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKE...

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DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an...

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Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a ...

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Thank you for your enquiry and patience. Our source for this protein has told me that yes, the protein can be reconstituted to a concentration of 1 mg/ml with water. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"