製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 領域416 to 766

特性

Our Abpromise guarantee covers the use of ab55690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: 74 nmol/min/mg using MEK1 as the substrate.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • FLJ95109
    • 94 kDa B raf protein
    • B raf 1
    • B Raf proto oncogene serine threonine protein kinase
    • B Raf proto oncogene, serine/threonine kinase
    • B RAF1
    • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
    • BRAF
    • BRAF 1
    • BRAF_HUMAN
    • BRAF1
    • cRmil
    • MGC126806
    • MGC138284
    • Murine sarcoma viral (v-raf) oncogene homolog B1
    • Murine sarcoma viral v raf oncogene homolog B1
    • NS7
    • Oncogen BRAF
    • oncogene BRAF1
    • p94
    • Proto-oncogene B-Raf
    • Proto-oncogene c-Rmil
    • RAFB 1
    • RAFB1
    • RMIL
    • Serine/threonine-protein kinase B-raf
    • v raf murine sarcoma viral oncogene homolog B
    • v raf murine sarcoma viral oncogene homolog B1
    • v-Raf murine sarcoma viral oncogene homolog B1
    see all
  • 機能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
  • 組織特異性Brain and testis.
  • 関連疾患Note=Defects in BRAF are found in a wide range of cancers.
    Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
    Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
    Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
    Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
    Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • 細胞内局在Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
  • Information by UniProt

Recombinant human B Raf protein 画像

  • Kinase activity plot using ab55690 with MEK1 as the substrate. Specific activity: 74 nmol/min/mg.
  • SDS-PAGE analysis of ab55690 with molecular weight markers. Apparent molecular weight: 63kDa

Recombinant human B Raf protein (ab55690) 使用論文

ab55690 has not yet been referenced specifically in any publications.

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