Recombinant human BRAF + Cdc37 protein (ab42596)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 25% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: Inhibition Assay
製品の詳細
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製品名
Recombinant human BRAF + Cdc37 protein -
生理活性
Specific Activity: 12 pmol/min/μg.
Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM
MgCl2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.
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精製度
> 25 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH -
タグ
His tag N-Terminus , DDDDK tag N-Terminus -
配列の追加情報
Human BRAF (NM_004333, a.a.432-725, N-term His-tag, 36 kDa), and p50 (NM_007065, a.a.2-378 (end), N-term FLAG-tag, 45 kDa). Protein exists in a complex with Cdc37 and p50.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab42596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Inhibition Assay
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製品の状態
Liquid -
備考
Coexpressed in Baculovirus infected Sf9 cell expression system.
Used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.53% Imidazole
Constituents: 0.018% Potassium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.7% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.72% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
B Raf: Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. Cdc37: Cytoplasm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab42596 は論文での使用が確認できていません。