The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
140U/mg activity. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 37 degrees C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant AXL.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.