Recombinant human Aurora B protein (ab51435)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • タグGST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab51435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考ab51435 was expressed in Sf9 insect cells.

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AIK2
    • AIM-1
    • AIM1
    • ARK-2
    • ARK2
    • AurB
    • AURKB
    • AURKB_HUMAN
    • Aurora 1
    • Aurora and Ipl1 like midbody associated protein 1
    • Aurora kinase B
    • Aurora related kinase 2
    • Aurora- and Ipl1-like midbody-associated protein 1
    • Aurora-B
    • Aurora-related kinase 2
    • Aurora/IPL1 related kinase 2
    • Aurora/IPL1-related kinase 2
    • IPL1
    • PPP1R48
    • Protein phosphatase 1 regulatory subunit 48
    • Serine/theronine kinase 12
    • Serine/threonine protein kinase 12
    • Serine/threonine-protein kinase 12
    • Serine/threonine-protein kinase aurora-B
    • STK-1
    • STK1
    • STK12
    • STK5
    see all
  • 機能May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • 組織特異性High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • 関連疾患Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • 細胞内局在Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt

Recombinant human Aurora B protein 画像

  • Kinase activity assay - specific activity 190nmol/min/mg
  • Recombinant human Aurora B (tagged). Molecular weight 68 kDa.

Recombinant human Aurora B protein (ab51435) 使用論文

ab51435 has not yet been referenced specifically in any publications.

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