The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
12 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 4 ug/ml Aurora B kinase.
備考Expressed in a Baculovirus infected Sf9 cell expression system.
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This product is an active protein and may elicit a biological response in vivo, handle with caution.
Aurora and Ipl1 like midbody associated protein 1
Aurora kinase B
Aurora related kinase 2
Aurora- and Ipl1-like midbody-associated protein 1
Aurora-related kinase 2
Aurora/IPL1 related kinase 2
Aurora/IPL1-related kinase 2
Protein phosphatase 1 regulatory subunit 48
Serine/theronine kinase 12
Serine/threonine protein kinase 12
Serine/threonine-protein kinase 12
Serine/threonine-protein kinase aurora-B
機能May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
組織特異性High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
関連疾患Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain.
翻訳後修飾Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
細胞内局在Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.