Recombinant Human AOS1+UBA2 protein (Chemical Free) (ab80730)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Human

特性

Our Abpromise guarantee covers the use of ab80730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Conjugation

    SDS-PAGE

  • 精製度
    > 90 % SDS-PAGE.

  • 製品の状態
    Lyophilised
  • 備考

    Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 50mM HEPES, 200mM Sodium chloride, 1mM DTT, pH 8.0

関連情報

  • 別名
    • SAE1
    • SAE2
    • SUMO 1 activating enzyme subunit 1
    • SUMO1 activating enzyme subunit 2
    • Ubiquitin-like 1-activating enzyme E1A
    • Ubiquitin-like 1-activating enzyme E1B
    • UBLE1A
    • UBLE1B
    see all
  • 関連性
    E1-SUMO protein is a heterodimer consisted of human AOS1 and UBA2. The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
  • 細胞内局在
    Nuclear

参考文献

ab80730 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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