Recombinant Human Androgen Receptor protein (ab82124)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus
  • アミノ酸配列
    • 生物種Human
    • 領域650 to 920
    • タグHis tag C-Terminus , DDDDK tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab82124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    EMSA

  • 精製度> 95 % SDS-PAGE.
    ab82124 is purified by an affinity chromatography in combination with FPLC.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

関連情報

  • 別名
    • AIS
    • ANDR_HUMAN
    • Androgen nuclear receptor variant 2
    • Androgen receptor
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease)
    • androgen receptor splice variant 4b
    • AR
    • AR8
    • DHTR
    • Dihydro testosterone receptor
    • Dihydrotestosterone receptor
    • Dihydrotestosterone receptor (DHTR)
    • HUMARA
    • HYSP1
    • KD
    • Kennedy disease (KD)
    • NR3C4
    • Nuclear receptor subfamily 3 group C member 4
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4)
    • SBMA
    • SMAX1
    • Spinal and bulbar muscular atrophy
    • Spinal and bulbar muscular atrophy (SBMA)
    • Testicular Feminization (TFM)
    • TFM
    see all
  • 機能Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • 組織特異性Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • 関連疾患Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • 翻訳後修飾Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 細胞内局在Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt

Recombinant Human Androgen Receptor protein (ab82124) 使用論文

ab82124 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. The Androgen Receptor ab82124 has a Flag tag on the N-terminus (I'll be sure to update the datasheet with this information) and a 8x His-tag on C terminus. There is no 6xH+GS sequence with this protein so I'm not sure...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"