製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Human
    • 配列
      MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK
    • 分子量
      20 kDa
    • 領域
      1 to 175

特性

Our Abpromise guarantee covers the use of ab48779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 精製度
    > 95 % SDS-PAGE.

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM Tris HCl, 1mM EDTA, pH 7.5

関連情報

  • 別名
    • AACRYA
    • Alpha B crystallin
    • Alpha crystallin B chain
    • Alpha(B)-crystallin
    • Alpha-crystallin B chain
    • CRYA2
    • Cryab
    • CRYAB_HUMAN
    • Crystallin alpha B
    • Crystallin alpha polypeptide 2
    • CTPP2
    • Heat shock 20 kD like protein
    • Heat shock protein beta 5
    • Heat shock protein beta-5
    • HspB5
    • Renal carcinoma antigen NY REN 27
    • Renal carcinoma antigen NY-REN-27
    • Rosenthal fiber component
    see all
  • 機能
    May contribute to the transparency and refractive index of the lens.
  • 組織特異性
    Lens as well as other tissues.
  • 関連疾患
    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • 配列類似性
    Belongs to the small heat shock protein (HSP20) family.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

画像

  • 14% SDS-PAGE gel loaded with ab48779,
    recombinant Human alpha B Crystallin,
    predicted molecular weight 20.1 kDa
  • Anti-Alpha B Crystallin antibody (ab85939) at 1 µg/ml + Recombinant Human Alpha B Crystallin protein (ab48779) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

参考文献

This product has been referenced in:
  • Kötter S  et al. Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. J Cell Biol 204:187-202 (2014). Read more (PubMed: 24421331) »
  • Rao NA  et al. Small Heat Shock Protein aA-Crystallin Prevents Photoreceptor Degeneration in Experimental Autoimmune Uveitis. PLoS One 7:e33582 (2012). Read more (PubMed: 22479415) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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