製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
    • 分子量
      55 kDa
    • 領域
      18 to 517

特性

Our Abpromise guarantee covers the use of ab87415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific activity is > 250pmol/min/ug, and was obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25C.

     

     

  • アプリケーション

    SDS-PAGE

    Functional Studies

    Western blot

  • エンドトキシン・レベル
    < 1.000 Eu/mg
  • 精製度
    > 90 % SDS-PAGE.
    ab87415 is purified using conventional chromatography techniques.
  • 製品の状態
    Liquid
  • 備考
    Endotoxin:< 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Acetaldehyde dehydrogenase 2
    • Aldehyde dehydrogenase 2 family
    • Aldehyde dehydrogenase 2 family (mitochondrial)
    • Aldehyde dehydrogenase mitochondrial
    • Aldehyde dehydrogenase, mitochondrial
    • ALDH 2
    • ALDH class 2
    • ALDH E2
    • ALDH-E2
    • Aldh2
    • ALDH2_HUMAN
    • ALDHI
    • ALDM
    • Liver mitochondrial ALDH
    • MGC1806
    • Mitochondrial aldehyde dehydrogenase 2
    • MS767
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2
    see all
  • パスウェイ
    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • 配列類似性
    Belongs to the aldehyde dehydrogenase family.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt

Recombinant human ALDH2 protein 画像

  • 15% SDS-PAGE showing 3µg ab87415 at approximately 54.5 kDa.
  • All lanes : Anti-ALDH2 antibody (ab70917) at 1/100 dilution

    Lane 1 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.1 µg
    Lane 2 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant human ALDH2 protein (ab87415) 使用論文

This product has been referenced in:
  • Yasgar A  et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). Read more (PubMed: 28129349) »

See 1 Publication for this product

Product Wall

Thank you for your reply. The specific information activity for these products is as follows:


ab87415: Specific activity is > 0.14 units/ml

ab93463 and ab117154: Specific activity is > 0.5 units/ml

The protocol used...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録