Recombinant human ALDH2 protein (Active) (ab87415)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/mg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
製品の詳細
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製品名
Recombinant human ALDH2 protein (Active)
ALDH2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25?.
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精製度
> 90 % SDS-PAGE.
ab87415 is purified using conventional chromatography techniques. -
エンドトキシン・レベル
< 1.000 Eu/mg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S -
予測される分子量
55 kDa -
領域
18 to 517 -
配列の追加情報
(NP_000681)
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab87415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Western blot
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製品の状態
Liquid -
備考
MW confirmed by MALDI-TOF.
Concentration determined by Absorbance at 280 nm.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Acetaldehyde dehydrogenase 2
- Aldehyde dehydrogenase 2 family
- Aldehyde dehydrogenase 2 family (mitochondrial)
see all -
パスウェイ
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
配列類似性
Belongs to the aldehyde dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab87415 は 1 報の論文で使用されています。
- Yasgar A et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). PubMed: 28129349