製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種
      Human
    • 分子量
      108 kDa
    • 配列の追加情報
      Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton

特性

Our Abpromise guarantee covers the use of ab73442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    ab73442 is reactive against Polymyositis sera.
  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

  • 精製度
    > 90 % SDS-PAGE.
    ab73442 is purified by proprietary chromatographic techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 250mM Sodium chloride, 16mM HEPES, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AARS
    • AI316495
    • Alanine tRNA ligase
    • Alanine tRNA ligase 1, cytoplasmic
    • Alanine tRNA ligase cytoplasmic
    • Alanine--tRNA ligase
    • Alanyl tRNA synthetase
    • Alanyl tRNA synthetase cytoplasmic
    • Alanyl-tRNA synthetase
    • AlaRS
    • C76919
    • CMT2N
    • cytoplasmic
    • EC 6.1.1.7
    • MGC37368
    • Renal carcinoma antigen NY REN 42
    • Renal carcinoma antigen NY-REN-42
    • SYAC_HUMAN
    see all
  • 機能
    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • 関連疾患
    Charcot-Marie-Tooth disease 2N
  • 配列類似性
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • ドメイン
    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • 翻訳後修飾
    ISGylated.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt

参考文献

ab73442 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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