製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q9Y243
    • 生物種Human
    • 配列MSPIDPMGHHHHHHGRRRASVAAGILVPRGSPGLDGICSIEEFSDVTIVK EGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAK CQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADR LQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGT FGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFL TSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSA LDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCG TPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELI LMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGV NWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMD CMDNERRPHFPQFSYSASGRE
    • 分子量60 kDa
    • 領域2 to 479
    • タグHis tag N-Terminus
    • 配列の追加情報Sequence includes His tag and linker (with the Thrombin cleavage site)

特性

Our Abpromise guarantee covers the use of ab79799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific Activity: 14 pmol/min/µg.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.05% Tween 20, 25mM Tris HCl, 138mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Akt3
    • AKT3 kinase
    • AKT3_HUMAN
    • DKFZp434N0250
    • MPPH
    • PKB gamma
    • PKBG
    • PRKBG
    • Protein kinase Akt-3
    • Protein Kinase AKT3
    • Protein kinase B gamma
    • RAC gamma
    • RAC gamma serine/threonine protein kinase
    • RAC-gamma serine/threonine-protein kinase
    • RAC-PK-gamma
    • RACPK Gamma
    • Serine threonine protein kinase Akt 3
    • Serine threonine protein kinase Akt3
    • STK 2
    • STK-2
    • STK2
    • V akt murine thymoma viral oncogene homolog 3
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma
    see all
  • 機能IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • 組織特異性In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • ドメインBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • 翻訳後修飾Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • 細胞内局在Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt

Recombinant human AKT3 protein 画像

  • 10% SDS-PAGE, Coomassie staining.
    Lane 1: ab79799 5µg.
    Lane 2: Protein marker.
  • Kinase Assay: Specific Activity 10 pmol/min/µg.

Recombinant human AKT3 protein (ab79799) 使用論文

ab79799 has not yet been referenced specifically in any publications.

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