Recombinant human AKT2 protein (ab79798)

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製品の概要

法規制情報

カルタヘナ法

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号P31751
    • 生物種Human
    • 配列MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E
    • 領域1 to 481
    • タグHis tag N-Terminus
    • 配列の追加情報Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.

特性

Our Abpromise guarantee covers the use of ab79798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: 5.9 pmol/min/µg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 精製度>90% by SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.1% Imidazole
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 50% Glycerol, 0.58% Sodium chloride, Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Akt2
    • AKT2_HUMAN
    • HIHGHH
    • murine thymoma viral (v-akt) homolog-2
    • PKB
    • PKB beta
    • PKBB
    • PKBBETA
    • PRKBB
    • Protein kinase Akt 2
    • Protein kinase Akt-2
    • Protein kinase B beta
    • rac protein kinase beta
    • RAC-BETA
    • RAC-beta serine/threonine-protein kinase
    • RAC-PK-beta
    • v akt murine thymoma viral oncogene homolog 2
    see all
  • 機能General protein kinase capable of phosphorylating several known proteins.
  • 組織特異性Expressed in all human cell types so far analyzed.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt

Recombinant human AKT2 protein 画像

  • Western blot - Active human AKT2 full length protein (ab79798)
    Western blot - Active human AKT2 full length protein (ab79798)
    Anti-AKT2 antibody (ab78666) + Recombinant human AKT2 protein (ab79798) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  • SDS-PAGE - AKT2 protein (Active) (ab79798)
    SDS-PAGE - AKT2 protein (Active) (ab79798)

    10% SDS-PAGE showing ab79798 at approximately 57kDa (10µg).

  • Functional Studies - AKT2 protein (Active) (ab79798)
    Functional Studies - AKT2 protein (Active) (ab79798)
    Kinase assay: Specific activity 5.9 pmol/min/μg.

Recombinant human AKT2 protein (ab79798) 使用論文

ab79798 has not yet been referenced specifically in any publications.

ab79798 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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