Recombinant human AKT2 protein (ab79798)

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製品の概要

法規制情報

カルタヘナ法

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
      P31751
    • 生物種
      Human
    • 配列
      MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E
    • 領域
      1 to 481
    • タグ
      His tag N-Terminus
    • 配列の追加情報
      Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.

特性

Our Abpromise guarantee covers the use of ab79798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific Activity: 5.9 pmol/min/µg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 精製度
    >90% by SDS-PAGE.

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.1% Imidazole
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 50% Glycerol, 0.58% Sodium chloride, Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Akt2
    • AKT2_HUMAN
    • HIHGHH
    • murine thymoma viral (v-akt) homolog-2
    • PKB
    • PKB beta
    • PKBB
    • PKBBETA
    • PRKBB
    • Protein kinase Akt 2
    • Protein kinase Akt-2
    • Protein kinase B beta
    • rac protein kinase beta
    • RAC-BETA
    • RAC-beta serine/threonine-protein kinase
    • RAC-PK-beta
    • v akt murine thymoma viral oncogene homolog 2
    see all
  • 機能
    General protein kinase capable of phosphorylating several known proteins.
  • 組織特異性
    Expressed in all human cell types so far analyzed.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt

画像

  • Western blot - Active human AKT2 full length protein (ab79798)
    Western blot - Active human AKT2 full length protein (ab79798)
    Anti-AKT2 antibody (ab78666) + Recombinant human AKT2 protein (ab79798) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  • SDS-PAGE - AKT2 protein (Active) (ab79798)
    SDS-PAGE - AKT2 protein (Active) (ab79798)

    10% SDS-PAGE showing ab79798 at approximately 57kDa (10µg).

  • Functional Studies - AKT2 protein (Active) (ab79798)
    Functional Studies - AKT2 protein (Active) (ab79798)
    Kinase assay: Specific activity 5.9 pmol/min/μg.

参考文献

ab79798 has not yet been referenced specifically in any publications.

ab79798 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1 Review or Q&A

10
Votes: Highest First

-Received vial over the weekend and warm,
-Do not want to try it.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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