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Our Abpromise guarantee covers the use of ab79792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The purified AKT1 is activated in vitro using PDK1 enzyme.
Specific Activity: 12.32 pmol/min/µg.
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate at room temperature for 1 hour.
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab79792 has not yet been referenced specifically in any publications.