Recombinant human AKR1B1 protein (ab117190)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQ NENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDL KLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVD EGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYC QSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLI RFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCA LLSCTSHKDYPFHEEF
    • 分子量
      36 kDa
    • 領域
      1 to 316

特性

Our Abpromise guarantee covers the use of ab117190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity : approximately 0.5 - 0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度
    > 95 % SDS-PAGE.
    ab117190 is purified by proprietary chromatographic techniques.
  • 製品の状態
    Liquid
  • 備考
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • ADR
    • AKR1B 1
    • Akr1b1
    • Aldehyde reductase
    • Aldehyde reductase 1
    • Aldo keto reductase family 1, member B1
    • Aldo-keto reductase family 1 member B1
    • aldo-keto reductase family 1, member B1 (aldose reductase)
    • Aldose reductase
    • aldr 1
    • ALDR_HUMAN
    • aldr1
    • ALR2
    • AR
    • Lii5 2 CTCL tumor antigen
    • Low Km aldose reductase
    • MGC1804
    see all
  • 機能
    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
  • 組織特異性
    Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
  • 配列類似性
    Belongs to the aldo/keto reductase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt

参考文献

ab117190 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your reply.

I spoke to the lab again and this time they were able to supply the raw data that you were asking for. This information is in the Excel file that is attached.

If there is anything else I can help you with, pl...

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Thank you for contacting Abcam.

I have talked to the lab and we are unable to send you the actual raw QC data, but below is the protocol they used to test the activity of ab117190:

Activity Assay:

1. Prepare a 1ml reaction mi...

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Thank you for contacting us today and your interest in our products.

I can now confirm that the specific activity figures I quoted to you over the phone did relate to the most current lot which you have received. Enzymatic activity was confir...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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