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Our Abpromise guarantee covers the use of ab52314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity:300,000 - 400,000 units/mg.
Enzyme Assay: Use at 1 unit/assay. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37degC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
This recombinant active human caspase 3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. It can be useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays.
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 15% Glycerol, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab52314 has not yet been referenced specifically in any publications.