Recombinant human active Caspase-2 protein (ab52098)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human active Caspase-2 protein -
生理活性
SPECIFIC ACTIVITY: 10,000 – 13,000 units/mg UNIT DEFINATION: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
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精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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特性
Our Abpromise guarantee covers the use of ab52098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 1082 Caspase-2, human recombinant. 1082-25 is the same size as the 25 unit size of ab52098.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
関連情報
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別名
- CASP2
- Caspase 2
- ICH 1L
see all -
関連性
Caspase 2 is involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. During apoptosis, procaspase-2 is processed at aspartate residues. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme. -
細胞内局在
Cytosol, membrane
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab52098 は論文での使用が確認できていません。