The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: > 60,000 unit/mg of protein.
Unit definition: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37oC in MES pH5.0 using 10mM of substrate.
% SDS-PAGE. ab87493 is purified using conventional chromatography techniques.
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.