Recombinant Human 14-3-3 zeta protein (ab87361)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human 14-3-3 zeta protein
14-3-3 zeta タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purified by using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN -
予測される分子量
32 kDa -
領域
1 to 245
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab87361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
関連情報
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別名
- 14 3 3 delta
- 14 3 3 protein zeta/delta
- 14 3 3 protein/cytosolic phospholipase A2
see all -
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
細胞内局在
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab87361 は 2 報の論文で使用されています。
- Ruff SE et al. PIM1 phosphorylation of the androgen receptor and 14-3-3 ? regulates gene transcription in prostate cancer. Commun Biol 4:1221 (2021). PubMed: 34697370
- Hou Y et al. Effect of cytokine-induced killer cells combined with dendritic cells on the survival rate and expression of 14-3-3? and p-Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16:1815-1820 (2018). PubMed: 30008870