The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. Store at -80°C.
Constituents: 0.1% Glycerol
The protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately. (Avoid repeated freeze/thaw cycles).
Transactivating regulatory protein
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
Recombinant HIV1 tat protein (ab109929) 使用論文
has not yet been referenced specifically in any publications.