Recombinant HIV1 Reverse Transcriptase protein (ab63979)

製品の概要

  • 製品名Recombinant HIV1 Reverse Transcriptase protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli

特性

Our Abpromise guarantee covers the use of ab63979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
  • アプリケーション

    SDS-PAGE

    Other

    Functional Studies

    Western blot

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • 備考ab63979 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.1% Triton-X-100, 50mM Sodium chloride, 5mM Magnesium chloride, 40mM Tris HCl, 10mM Beta mercaptoethanol, pH 8.3

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • HIV 1 Reverse transcriptase
    • HIV1 RT
    • Human Immunodeficiency virus 1
    • p51 RT
    • p66 RT
    • Reverse transcriptase p51 subunit
    • Reverse transcriptase p66 subunit
    see all
  • 関連性HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.

Recombinant HIV1 Reverse Transcriptase protein 画像

  • Polyacrylamide gel electrophoresis of ab63979.
  • Anti-HIV1 Reverse Transcriptase antibody (ab63911) at 1/2500 dilution + Recombinant HIV1 Reverse Transcriptase protein (ab63979) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

Recombinant HIV1 Reverse Transcriptase protein (ab63979) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall



Yes there are 200 units/vial.

Thank you for contacting us.
This can vary from lot to lot, but 1 vial of the current lot of HIV1 reverse transcriptase protein is 10 ug in 50 ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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ab63979 HIV1 Reverse Transcriptase protein has been functionally tested as noted on the online datasheet, which reads

"Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole in...

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Thank you for contacting Abcam regarding this product.
I have had the opportunity to speak with the lab regarding the specific activity of this product. Their response is as follows:
Definition of activity: Activity of intake of 1 nmole of ...

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Thank you for contacting Abcam.

I have heard back from the lab and they have told me that the protein concentration was measured by BCA method using assay kit from Pierce and the concentration was also confirmed by OD280. They measured the co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"