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Our Abpromise guarantee covers the use of ab63995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab63995 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
Active as HIV-1 protease substrate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 20% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 7.5
SDS-PAGE analysis of Recombinant HIV1 p55 protein. As the substrate, Recombinant HIV1 p55 protein (1 μg, ab63995) was used in 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. Lane 1: no protease,
Lane 2: 0.16 pg.
Lane 3: 1.6 pg.
Lane 4: 16 pg
Lane 5: 0.16 μg
Lane 6: 1.6 μg protease.
Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.
ab63995 has not yet been referenced specifically in any publications.