製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 分子量26 kDa

特性

Our Abpromise guarantee covers the use of ab43037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Immunoreactive with all sera of HIV1 infected individuals.
  • アプリケーション

    ELISA

    Western blot

  • 精製度> 95 % SDS-PAGE.
    Organic extraction > S-Sepharose > S-300.
  • 製品の状態Liquid
  • 備考

    Immunoreactive with all sera of HIV-1 infected individuals.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 8M Urea, 50mM Tris, pH 7.2

関連情報

  • 別名
    • CA
    • Capsid protein p24
    • HIV-1 Gag p24
    • HIV1gp1
    • Human immunodeficiency virus 1
    • Human immunodeficiency virus type 1 p24
    see all
  • 関連性HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • 細胞内局在Membrane

Recombinant HIV1 p24 protein (ab43037) 使用論文

This product has been referenced in:
  • Rosemary Bastian A  et al. Mechanism of multivalent nanoparticle encounter with HIV-1 for potency enhancement of peptide triazole virus inactivation. J Biol Chem 290:529-43 (2015). Read more (PubMed: 25371202) »
  • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). SDS-PAGE . Read more (PubMed: 23223527) »

See all 2 Publications for this product

Product Wall

Unfortunately we are unable to share the specific sequence but it is available in the literature. The protein is derived from group MN. These regions are highly conserved throughout all strains.

Thank you for contacting us.


The immune-biological activity of HIV p24 is derived from its linear AA sequences and has no secondary structure related activity. This protein has not been tested for functional activity.

I hope...

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You are correct, ab43037 is an antigen, not an antibody, and it is suitable to be coated on an ELISA plate from 100 ng to 10 ug. The dilution buffer that you use does not have to contain urea, as long as you are coating the wells within the range given...

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ab43037 works well in ELISA at nano to tens of micro gram levels. Thus, dilutions within that range should work sufficiently.

Thank you for contacting us.

The laboratory provided the following ranges for ELISA. Using ab43037 protein use 100 ng to 10 ug to coat the plate, detection with ab81433 use at 1/5000-1/10000.

I hope this information is helpful to ...

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Thank you for contacting us.
I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELI...

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Thank you for contacting us.

Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

http://www.abcam.com/index.html?datasheet=9071 (or use the following: http://www.abcam.com/index.ht...

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Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test re...

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Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not...

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Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. http://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"