The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
One unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-
Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 per minute at pH 7.5 at 25° C. ab123540 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Hepatitis Virus Genotype 1b NS3
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein.
Recombinant Hepatitis Virus Genotype 1b NS3 protein (ab123540) 使用論文
has not yet been referenced specifically in any publications.