Recombinant Hepatitis C Virus NS3 protein (Biotin) (ab68047)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 領域1450 to 1643
    • 配列の追加情報The E.coli derived recombinant Biotin Labeled protein contains the HCV NS3 immunodominant regions. The Biotin labeled protein is fused with b-galactosidase (114 kDa) at N-terminus.
  • 標識Biotin

特性

Our Abpromise guarantee covers the use of ab68047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性ab68047 is immunoreactive with sera of HCV-infected individuals.
  • アプリケーション

    Western blot

    ELISA

  • 精製度> 95 % SDS-PAGE.
    ab68047 was purified by a proprietary chromatographic technique.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • 関連性HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • 細胞内局在Endoplasmic reticulum membrane

Recombinant Hepatitis C Virus NS3 protein (Biotin) (ab68047) 使用論文

ab68047 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Please accept my apologies for the delay, it was a very comprehensive search.

Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"