Recombinant Hepatitis C Virus NS3 protein (ab49025)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, Flow Cyt, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Hepatitis C Virus NS3 protein
Hepatitis C Virus NS3 タンパク質・ペプチド 製品一覧 -
生理活性
Reactivity with human HCV positive serum undetermined.
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精製度
> 95 % SDS-PAGE.
Purity of proteins is evaluated by SDS-PAGE -
発現系
Escherichia coli -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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領域
1450 to 1643
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特性
Our Abpromise guarantee covers the use of ab49025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Flow Cytometry
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl, 50% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Hepatitis C Virus NS3
- NS3
- NS3P
see all -
関連性
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
細胞内局在
Endoplasmic reticulum membrane
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab49025 は論文での使用が確認できていません。