The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性One unit of protease hydrolyzes 1 picomole of Ac-Asp-Glu-Dap(QXL™520)-
Glu-Glu-Abu-COO-Ala -Ser-Cys(5-FAMsp)-NH2 per minute at pH 7.5 at 25° C. ab123527 is in active form and the pre-activation by pep4A or pep4AK is not necessary.
NS3 protease of hepatitis C virus (HCV), located on the N-terminal domain of HCV NS3, is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. The HCV NS3 is a chymotrypsin-like serine protease. It requires a cofactor, a 54 amino acid NS4 protein, to reach its optimal activity. The X-ray crystal structure studies show that NS3 forms a tight non-covalent complex with NS4. The NS3/4A protease is essential for viral replication and the formation of infectious viral particles.
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
HCV Core 3a
HCV Core genotype 3a
Hepatitis C Virus Core genotype 3a
関連性HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression.
HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
Recombinant Hepatitis C Virus Core 3a protein (ab123527) 使用論文
has not yet been referenced specifically in any publications.