Recombinant Human H-Ras (G12V) protein (Tagged) (ab90627)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant Human H-Ras (G12V) protein (Tagged)
H-Ras (G12V) タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS -
予測される分子量
21 kDa -
領域
1 to 189 -
修飾
mutated G12V -
タグ
GST tag N-Terminus -
配列の追加情報
(NP_005334)
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab90627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
The mutation G12V leads to elimination of the intrinsic GTPase activity.
ab90627 is effective in activation of PI3K and PKB.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.87% Sodium chloride, 0.395% Tris HCl, 0.039% Beta mercaptoethanol, 0.0475% Magnesium chloride
関連情報
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別名
- c-H-ras
- CBAS/HAS
- CHARAS1
see all -
機能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
組織特異性
Widely expressed. -
関連疾患
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
翻訳後修飾
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. -
細胞内局在
Cell membrane. Cell membrane. Golgi apparatus. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus and Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Colocalizes with GNB2L1 to the perinuclear region. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab90627 は 1 報の論文で使用されています。
- Kovalski JR et al. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Mol Cell 73:830-844.e12 (2019). PubMed: 30639242