Recombinant rat CXCL1/GRO alpha protein (ab9773)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
-
製品名
Recombinant rat CXCL1/GRO alpha protein
CXCL1/GRO alpha タンパク質・ペプチド 製品一覧 -
精製度
> 98 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per g (1EU/g). -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Rat -
配列
APVANELRCQCLQTVAGIHFKNIQSLKVMP PGPHCTQTEVIATLKNGREACLDPEAPMVQ KIVQKMLKGVPK -
予測される分子量
11 kDa -
領域
25 to 96
-
特性
Our Abpromise guarantee covers the use of ab9773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
Western blot
-
製品の状態
Lyophilized -
備考
The maximal chemotactic activity of rat CXCL1/GRO alpha on rat neutrophils was determined by its ability to chemoattract rat neutrophils using a concentration range of 10.0-100.0 ng/ml.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成For lot specific reconstitution information please contact our Scientific Support Team.
関連情報
-
別名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
機能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
画像
-
All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.1 µg
Lane 2 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab9773 は論文での使用が確認できていません。