Recombinant Firefly Firefly Luciferase protein (ab100961)

製品の概要

  • 製品名Recombinant Firefly Firefly Luciferase protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P08659
    • 生物種Firefly (Photinus pyralis)
    • 配列MGSSHHHHHHSSGLVPRGSHMMEDAKNIKKGPAPFYPLEDGTAGEQLHKA MKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIV VCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVS KKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEY DFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGN QIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQD YKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAK RFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTG KTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDED EHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGE LPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGK LDARKIREILIKAKKGGKIAV
    • 分子量63 kDa including tags
    • 領域1 to 550
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab100961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度> 90 % SDS-PAGE.
    ab100961 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

関連情報

  • 別名
    • ec 1.13.12.7
    • Firefly
    • Luciferase
    • Luciferin 4 monooxygenase
  • 関連性Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • 細胞内局在Peroxisome

Recombinant Firefly Firefly Luciferase protein 画像

  • 15% SDS-PAGE showing ab100691 (3 µg) at approximately 62.9 kDa.

Recombinant Firefly Firefly Luciferase protein (ab100961) 使用論文

ab100961 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Our EasyLink conjugation kits are used to conjugate prepared enzymatic, fluorescent or biotin/streptavidin conjugates to antibodies.

http://www.abcam.com/index.html?pageconfig=resource&rid=13148#enz...

Read More

Thank you for your inquiry. The MSDS is linked to each online datasheet where the product requires one.  It is on the right hand side of the page under the shipping and pricing information (under the "Datasheet PDF" link). For ab290: http:/...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"