Recombinant E. coli Thioredoxin / TRX protein (ab115709)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P0AA25
    • 生物種Escherichia coli
    • 分子量12 kDa
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab115709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    ab115709 is purified by multi-step chromatography
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.58% Sodium chloride, 0.03% EDTA

関連情報

  • 別名
    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • 機能Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 配列類似性Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • 翻訳後修飾In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • 細胞内局在Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

Recombinant E. coli Thioredoxin / TRX protein 画像

  • Thioredoxin /TRX antibody + Recombinant E. coli Thioredoxin / TRX protein (ab115709) at 0.1 µg
  • SDS-PAGE analysis
    Lane 1: MW markers
    Lane 2: ab115709 at 1 µg
    Lane 3: ab115709 at 2 µg
    Lane 4: ab115709 at 5 µg

Recombinant E. coli Thioredoxin / TRX protein (ab115709) 使用論文

ab115709 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115709.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"