Recombinant E. coli Thioredoxin / TRX protein (ab115709)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Escherichia coli
    • 分子量
      12 kDa
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab115709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

    Functional Studies

  • 精製度
    > 95 % SDS-PAGE.
    ab115709 is purified by multi-step chromatography
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.58% Sodium chloride, 0.03% EDTA

関連情報

  • 別名
    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • 機能
    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 配列類似性
    Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • 翻訳後修飾
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • 細胞内局在
    Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

画像

  • Thioredoxin /TRX antibody + Recombinant E. coli Thioredoxin / TRX protein (ab115709) at 0.1 µg
  • SDS-PAGE analysis
    Lane 1: MW markers
    Lane 2: ab115709 at 1 µg
    Lane 3: ab115709 at 2 µg
    Lane 4: ab115709 at 5 µg

参考文献

ab115709 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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