製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Escherichia coli
    • 配列MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALE GHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAET DQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHG HVHGAHDHHHDHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH

特性

Our Abpromise guarantee covers the use of ab72847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant SlyD protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.
    ab72847 is purified by conventional chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM Tris, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • FKBP type peptidyl prolyl cis trans isomerase slyD
    • Histidine rich protein
    • PPIase
    • Rotamase
    • WHP
    see all
  • 関連性slyD belongs to the FKBP-type PPIase family.Required for lysis of phiX174 infected cells. It binds nickel and zinc with high affinity and 1:1 stoichiometry, copper and cobalt with lower affinity. No binding is detectable for ferrous, ferric, magnesium and calcium ions. The activity of slyD is considerably smaller than the one found in other PPIases with the same substrate. The substrate specificity carried out with 'suc-Ala-Xaa-Pro-Phe-4NA', where Xaa is the AA tested, was found to be Phe > Ala > Leu.
  • 細胞内局在Cytoplasmic

Recombinant E. coli slyD protein 画像

  • SDS-PAGE showing ab72847 (3µg) at approximately 21kDa.

Recombinant E. coli slyD protein (ab72847) 使用論文

ab72847 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"