Recombinant E. coli RuvB protein (Active) (ab63827)

製品の概要

  • 製品名Recombinant E. coli RuvB protein (Active)
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Escherichia coli

特性

Our Abpromise guarantee covers the use of ab63827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

    Western blot

    ELISA

  • 精製度> 90 % SDS-PAGE.
    purified by methods such as chromatography
  • 製品の状態Liquid
  • 備考

    This protein can be used for:- 1) Studies on homologous recombination mechanism. 2) To make use of the motor protein function that specifically migrates the Holliday junction by forming a complex with RuvA (branch-migration protein).

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 5mM Beta mercaptoethanol, 2mM EDTA, 100mM Sodium chloride, 10mM Tris HCl, pH 7.5

関連情報

  • 別名
    • ATP dependent DNA helicase, component of RuvABC resolvasome
    • Holliday junction ATP dependent DNA helicase ruvB
    • Holliday junction helicase subunit A
    • RUVB (recombination protein) homolog
  • 関連性In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC,RuvAB is uncoupled from RuvC and DSBs may be prevented.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear

Recombinant E. coli RuvB protein (Active) 画像

  • SDS Page analysis of ab63827

Recombinant E. coli RuvB protein (Active) (ab63827) 使用論文

ab63827 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"