Recombinant E. coli RNase H protein (ab113149)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P0A7Y4
    • 生物種Escherichia coli
    • 分子量20 kDa including tags
    • 領域1 to 155
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab113149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.
    ab113149 was purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • 備考


  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.04% DTT

関連情報

  • 別名
    • Ribonuclease H
    • Ribonuclease HI
    • RNase H
    • RNase HI
    • rnhA
    see all
  • 関連性RNase H from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in RNA:DNA duplexes to generate products with 3'-hydroxyl and 5'-phosphate ends.1,2,3 RNase H degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand). Other enzymes in E. coli which degrade RNA in the DNA-RNA hybrid are DNA polymerase I and exonuclease III, but these degrade either the RNA or DNA of the hybrids. Ribonuclease H will not cleave single-stranded or double-stranded DNA or RNA.1,2
  • 細胞内局在Cytoplasmic

Recombinant E. coli RNase H protein 画像

  • 15% SDS-PAGE showing ab113149 (3 µg) at approximately 20 kDa.

Recombinant E. coli RNase H protein (ab113149) 使用論文

ab113149 has not yet been referenced specifically in any publications.

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