Recombinant E. coli RecQ DNA Helicase protein (ab63813)

製品の概要

  • 製品名Recombinant E. coli RecQ DNA Helicase protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P15043
    • 生物種Escherichia coli
    • 配列MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR AHVDGDDEE

特性

Our Abpromise guarantee covers the use of ab63813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Unwinding duplex DNA, dependent on ATP. DNA-dependent ATPase.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.
    highly purified by several steps of chromatography
  • 製品の状態Liquid
  • 備考


    This protein can be used for: 1) Studies on mechanisms of DNA recombination, replication and repair. 2) Application for genetic engineering; For dissociation of special structure DNA.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 50mM Potassium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • ATP dependent DNA helicase
    • ATP dependent DNA helicase recQ
    • DNA helicase
    • ECK3816
    • JW5855
    • RecQ
    see all
  • 関連性RecQ is a DNA helicase, involved in the recF recombination pathway; its gene expression is under the regulation of the SOS system.
  • 細胞内局在Cytoplasmic, Bacterial nucleoid, Replisome

Recombinant E. coli RecQ DNA Helicase protein 画像

  • SDS Page analysis of RecQ DNA Helicase (ab63813)

Recombinant E. coli RecQ DNA Helicase protein (ab63813) 使用論文

This product has been referenced in:
  • Khan I  et al. Impact of age-associated cyclopurine lesions on DNA repair helicases. PLoS One 9:e113293 (2014). Read more (PubMed: 25409515) »
  • Suhasini AN  et al. DNA repair and replication fork helicases are differentially affected by alkyl phosphotriester lesion. J Biol Chem 287:19188-98 (2012). Read more (PubMed: 22500020) »

See all 3 Publications for this product

Product Wall

The RecQ helicase is full-length recombinant protein without any tag, expressed in E.coli. The earliest reference on the datasheet describes production of the protein.

Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS sign...

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I have contacted the lab regarding your enquiry. They have confirmed that RecQ helicase activity will depend on structures of DNA duplex.

The following paper describes details of assay conditions with various forms of DNA substrates ...

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Thank you for contacting us.

This protein corresponds to the Swissprot ID P15043. http://www.uniprot.org/uniprot/P15043

The molecular weight is 68kDa for unprocessed protein. The observed molecular weight as seen in image is 64 kda...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"