The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: 1 nmol of ab91349 will reduce 0.6 nmol peptide bound Met-R-sulfoxide in 1 min at 37°C. MetO-containing peptides are reduced by the enzyme in the presence of DTT.
% SDS-PAGE. NiNTA purified
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 33% Glycerol, Tris HCl, pH 7.4
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Methionine sulfoxide reductase B
Peptide methionine (R) S oxide reductase
Peptide methionine sulfoxide reductase msrB
Like most bacteria, Escherichia coli expresses a single msrB (Methionine-R-sulfoxide reductase) that carries out the reduction of methionine-R-sulfoxide to methionine. The E. coli enzyme belongs to a group of MsrBs, that possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.
SDS-PAGE - msrB protein (Active) (ab91349)
1 µg ab91349 on 12% SDS-PAGE gel
has not yet been referenced specifically in any publications.