製品の概要

  • 製品名
    Recombinant E. coli LexA protein
  • タンパク質長
    Full length protein

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Escherichia coli
    • 配列
      MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL ARKGVIEIVS GASRGIRLLQ EEEEGLPLVG RVAAGEPLLA QQHIEGHYQV DPSLFKPNAD FLLRVSGMSM KDIGIMDGDL LAVHKTQDVR NGQVVVARID DEVTVKRLKK QGNKVELLPE NSEFKPIVVD LRQQSFTIEG LAVGVIRNGD WL

特性

Our Abpromise guarantee covers the use of ab63816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度
    > 90 % SDS-PAGE.
    highly purified by several steps of chromatography
  • 製品の状態
    Liquid
  • 備考


    This protein can be used in: 1) Studies on the mechanism of E. coli SOS response. 2) Used as an antigen for positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleus as protein of the expected size in the yeast two-hybrid method using the lexA gene.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 5mM Beta mercaptoethanol, 2mM EDTA, 100mM Sodium chloride, 10mM Tris HCl, pH 7.5

関連情報

  • 別名
    • Lex A
    • LexA repressor
  • 関連性
    E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence (TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and DNA repair ability and mutagenic activity in the cells are enhanced.

画像

  • SDS-PAGE analysis of Recombinant E. coli LexA protein (ab63816).

  • SDS Page analysis of ab63816

参考文献

This product has been referenced in:
  • Bunnell BE  et al. Zinc blocks SOS-induced antibiotic resistance via inhibition of RecA in Escherichia coli. PLoS One 12:e0178303 (2017). Read more (PubMed: 28542496) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab63816.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録