Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Escherichia coli
    • 配列
      MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

特性

Our Abpromise guarantee covers the use of ab87414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度
    > 90 % SDS-PAGE.
    Purified by conventional chromatography techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Glycerol, 50mM MES, 2mM EDTA, 0.5mM DTT, 0.1mM PMSF, pH 6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • G6PD
    • G6PD_HUMAN
    • G6PD1
    • G6pdx
    • Glucose 6 phosphate 1 dehydrogenase
    • Glucose 6 phosphate dehydrogenase
    • Glucose 6 phosphate dehydrogenase, G6PD
    • Glucose-6-phosphate 1-dehydrogenase
    • MET19
    • POS10
    • Zwf1p
    see all
  • 機能
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 組織特異性
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • パスウェイ
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 関連疾患
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 配列類似性
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻訳後修飾
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt

画像

  • ab87414 on 15% SDS-PAGE (4µg)

参考文献

ab87414 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry.

I can confirm that ab87414 is a recombinant full length E coli Glucose 6 Phosphate Dehydrogenase and it is not tagged.

If you need any further assistance in the future, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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