Recombinant Dengue Virus 2 NS1 glycoprotein (ab64456)



  • 由来
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Dengue virus 2
    • 分子量
      48 kDa including tags
    • タグ
      His tag C-Terminus


Our Abpromise guarantee covers the use of ab64456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション


    Western blot


  • 精製度
    > 90 % SDS-PAGE.

  • 製品の状態
  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA


  • 別名
    • Dengue NS1
    • Dengue Virus non structural protein 1
  • 関連性
    NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.



This product has been referenced in:
  • Chen J  et al. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog 11:e1005053 (2015). Read more (PubMed: 26226614) »

See 1 Publication for this product

レビューと Q&A

This protein is purified by affinity chromatography.

On a gel, it can be seen as a monomer, dimer and polymer. We usually recommend heating samples up to 95ºC in SDS-Page before loading and running the gel in reducing conditions.
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Thank you for your new contact.

As I have explained in my previous e-mail, ab64456 has never been used as immunogen.

Since this glycoprotein is epitope tagged (having additional amino acids ie.HA sequence: YPYDVPDYA) and the storage...

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Thank you for your interest in our products.

The two kits we have for detection of Dengue virus-specific IgG or IgM ab108728 and ab108729, are for detection of antibodies in serological samples that detect Dengue virus. So, they detect an imm...

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Thank you for your enquiry regarding ab64456.

It is a Dengue Virus NS1 glycoprotein protein (His tag) which has been tested and characterised for ELISA and WB applications. It has not been used as immunogen to raise an antibody against.

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Thank you for contacting us. I apologize for the delay.

The ab64456 Dengue NS1is from serotype 2, Thailand strain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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My customer would like to know the strain name of Dengue virus protein is from. Could you provide this info?

Many thanks in advance!

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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Gracias por tu respuesta.
He consultado esta información con mis compañeros del laboratorio.
La glicoproteína (ab64456) contiene toda la secuencia excepto el dominio transmembranal. Además contiene 2x6 His tags, uno localizado en el extremo N...

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Thank you for contacting us.
I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, b...

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I am sorry for this misunderstanding.

We do not know which protein ab9200 exactly recognises and therefore I suspect this antibody will not be suitable for your purposes.

We onlyhave a mouse monoclonalantibody against Dengue Virus N...

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