Recombinant Cyclophilin B protein (ab53870)

製品の概要

• 製品名
  Recombinant Cyclophilin B protein
  Cyclophilin B タンパク質・ペプチド 製品一覧
• タンパク質長
  Protein fragment

製品の詳細

• 由来
  Recombinant
• 由来
  Escherichia coli

特性

• 生理活性
  Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

  Activity Assay

  1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
  2. Add 10 µl of recombinant Cyclophilin B protein with 1 µg in assay buffer.
  3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
  4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
  5. Record the increase in A405 nm for 30 minutes at 25°C.
• アプリケーション

  SDS-PAGE

  Functional Studies

• エンドトキシン・レベル
  < 1.000 Eu/µg
• 質量分析
  MALDI-TOF
• 精製度
  > 95 % SDS-PAGE.
  This product was expressed in E.coli and purified by using conventional chromatography techniques.
  
• 製品の状態
  Liquid
• Concentration information loading...

前処理および保存

• 保存方法および安定性

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  Preservative: None
  Constituents: 10% Glycerol, 20mM Sodium chloride, 20mM Tris, 0.5mM DTT, pH 8.0

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

Recombinant Cyclophilin B protein 画像

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  SDS-PAGE - Cyclophilin B protein (ab53870)
  ab53870 on 15% SDS-PAGE (3?)