製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli

関連製品

特性

Our Abpromise guarantee covers the use of ab53870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant Cyclophilin B protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル
    < 1.000 Eu/µg
  • 質量分析
    MALDI-TOF
  • 精製度
    > 95 % SDS-PAGE.
    This product was expressed in E.coli and purified by using conventional chromatography techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Sodium chloride, 20mM Tris, 0.5mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AA408962
    • AA553318
    • AI844835
    • Cphn 2
    • Cphn2
    • Cyclophilin B
    • Cyclophilin like protein
    • CyP 20b
    • CYP S1
    • CYP-S1
    • CYPB
    • EC 5.2.1.8
    • MGC14109
    • MGC2224
    • OI9
    • peptidyl prolyl cis trans isomerase B
    • Peptidyl prolyl cis trans isomerase B precursor
    • Peptidyl-prolyl cis-trans isomerase B
    • Peptidylprolyl isomerase B
    • peptidylprolyl isomerase B (cyclophilin B)
    • PPIase
    • PPIase B
    • Ppib
    • PPIB_HUMAN
    • Rotamase
    • Rotamase B
    • S cyclophilin
    • S-cyclophilin
    • SCYLP
    see all
  • 機能
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 関連疾患
    Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
  • 配列類似性
    Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • 細胞内局在
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

画像

  • SDS-PAGE - Recombinant Cyclophilin B protein (ab53870)
    SDS-PAGE - Recombinant Cyclophilin B protein (ab53870)

    ab53870 on 15% SDS-PAGE (3μg)

参考文献

This product has been referenced in:
  • Watanabe A  et al. CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. J Virol 84:4183-93 (2010). Read more (PubMed: 20147391) »

See 1 Publication for this product

ab53870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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