The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: 1,000 units/mg (1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute).
Bovine PI 3-Kinase alpha differs from the Human enzyme in only 2 positions, K532R and S535C. Both lie in the PIK domain of the enzyme (aa 525-696) and are not expected to interfere with binding of p85 (aa 31-108) or Ras (aa 173-292) or with catalytic function (aa 699-1064).
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3´ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III.
All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110 kDa) and a non-catalytic subunit (MW 50, 55, 85, or 101 kDa) and are known to phosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2) in vitro. The class I members can be further subdivided into class IA and IB PI3Ks. Class IA exists in three isoforms (p110alpha, p110beta and p110delta whereas the only class IB member is termed p110gamma.
Class IA PI3Ks are activated by adaptor proteins such as Ras or BCAP, or tyrosine-kinase-associated receptors including antigen, co-stimulatory and cytokine receptors (e.g. CD19, CD28, Insulin receptor, EGFR, and PDGFR). p110gamma is activated by G-protein-coupled receptors (GPCRs). Effectors of class I PI3Ks are pleckstrin homology domain proteins such as Akt/PKB, BTK, TEC, ITK, BAM32, and small GTPases (e.g. Cdc42, Rac, or Ras). The action of PI3Ks is regulated by the phosphatidylinositol-
3,4,5-trisphosphate phosphatases SHIP and PTEN.
SDS-PAGE - PI 3 Kinase p85 alpha protein (Active) (ab91351)
SDS-PAGE analysis of ab91351
has not yet been referenced specifically in any publications.