Recombinant Arginine deiminase protein (ab119147)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant Arginine deiminase protein
Arginine deiminase タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml. -
精製度
> 95 % SDS-PAGE.
Purity greater than 97% by SDS-PAGE gel and HPLC analyses. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mycoplasma arginini -
配列
SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILES HDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLED SEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVD PMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTP WYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKEC EFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVN GGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGT NYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSM PLSRKDVKW -
予測される分子量
46 kDa -
領域
2 to 410
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特性
Our Abpromise guarantee covers the use of ab119147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
備考
Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.003% DTT, 0.03% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
関連情報
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別名
- AD
- ADI
- Arginine deiminase
see all -
関連性
Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2. -
細胞内局在
Cytoplasmic
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab119147 は論文での使用が確認できていません。