The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: >10 unit/mg solid.
This protein is suitable for the determination of alpha-amylase in clinical analysis.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: EDTA, Lactose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Immediately prior to use, prepare stock solution of 5mg solid/ml in cold 10mM potassium phosphate. Immediately dilute 0.1ml into 15.1 ml 10mM potassium phosphate.
Glucose phosphomutase 1
ß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate.
This enzyme participates in both the breakdown and synthesis of glucose.
Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively.
ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
has not yet been referenced specifically in any publications.