製品の概要

  • 製品名Anti-RECA1 antibody [HIS52]
  • 製品の詳細
    Mouse monoclonal [HIS52] to RECA1
  • 特異性This antibody recognises RecA-1, a cell surface antigen which is expressed by all rat endothelial cells.
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat
    非交差種: Mouse, Sheep, Rabbit, Goat, Chicken, Guinea pig, Pig
  • 免疫原

    Tissue/ cell preparation (Rat). Stromal cells from rat lymph node

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

Anti-RECA1 antibody [HIS52] (ab22492) 使用論文

This product has been referenced in:
  • Wu CC  et al. Human umbilical vein endothelial cells protect against hypoxic-ischemic damage in neonatal brain via stromal cell-derived factor 1/C-X-C chemokine receptor type 4. Stroke 44:1402-9 (2013). Read more (PubMed: 23449265) »
  • Cantinieaux D  et al. Conditioned medium from bone marrow-derived mesenchymal stem cells improves recovery after spinal cord injury in rats: an original strategy to avoid cell transplantation. PLoS One 8:e69515 (2013). Read more (PubMed: 24013448) »

See all 7 Publications for this product

Product Wall

Thank you for your query.

The epitope recognised by ab22492 is reported to be sensitive to formaldehyde fixation (which is commonly used in IHC-P). There are publications which have used this clone successfully in IHC-P but we have never been...

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Thank you for contacting us.

ab9774 is a well characterized antibody is fully guaranteed to detect the endothelial cells. Please use the product without any hesitation.

ab22492 is also reactive against vascular endothelium so this pro...

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Have agood week too.

Do not hesitate to contact us again if you need any more advice or information.



Thank you very much for this confirmation. I have added one unit of anti-RECA1 ab22492 (free of charge replacement for ab9774)to the next orderto ****.

Thank you for your reply and this precision.

I have just realised that ab9774 is not an anti-RECA1 antibody. RECA1 is the clone number of this anti-Endothelial Cell antibody.

The only 2 anti-RECA1 antibodies we have in our catalog a...

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Thank you for contacting us and sorry for the delay of our reply.

After looking at our archives, we are confident that Lee et al. ordered andused ab22492, not ab9774.

Regarding the IHC, the publication mention a use of the antibody...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"