Anti-Rec-8 abnormal REcombination 抗体 (ab52792)

製品の概要

  • 製品名Anti-Rec-8 abnormal REcombination antibody
  • 製品の詳細
    Mouse polyclonal to Rec-8 abnormal REcombination
  • アプリケーション適用あり: WB, IHC-FoFrmore details
  • 種交差性
    交差種: Caenorhabditis elegans
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to N terminal amino acids 647-746 of Caenorhabditis elegans Rec-8 abnormal REcombination. Target sequence used to make the antibody: MRTEEELENV RRRQKSSLGV QFMRTDDLEE DTRRNRLFED EERTRDARED ELFFYSSGSL LPNNRLNIHK ELLNEAEARY PEWVNFNEFT ADHDRKKAAT.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 90 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

IHC-FoFr 1/200. PubMed: 19696886

ターゲット情報

  • 関連性Rec-8 is a Rad21/Rec8 like protein. This family represents a conserved N-terminal region found in eukaryotic cohesins of the Rad21, Rec8 and Scc1 families.
  • 参照データベース
    • 別名
      • Abnormal REcombination antibody
      • rec 8 antibody

    Anti-Rec-8 abnormal REcombination antibody 画像

    • All lanes : Anti-Rec-8 abnormal REcombination antibody (ab52792) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 90 kDa

    Anti-Rec-8 abnormal REcombination antibody (ab52792) 使用論文

    This product has been referenced in:
    • Shakes DC  et al. Spermatogenesis-specific features of the meiotic program in Caenorhabditis elegans. PLoS Genet 5:e1000611 (2009). IHC-FoFr ; Caenorhabditis elegans . Read more (PubMed: 19696886) »

    See 1 Publication for this product

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