Anti-REA 抗体 (ab71970)
Key features and details
- Rabbit polyclonal to REA
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-REA antibody
REA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to REA -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human REA.
Database link: Q99623 -
ポジティブ・コントロール
- WB: HeLa, HEK-293T, NIH/3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71970の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. -
配列類似性
Belongs to the prohibitin family. -
発生段階
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
細胞内局在
Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 515363 Cow
- Entrez Gene: 11331 Human
- Entrez Gene: 12034 Mouse
- Entrez Gene: 114766 Rat
- Omim: 610704 Human
- SwissProt: Q2HJ97 Cow
- SwissProt: Q99623 Human
- SwissProt: O35129 Mouse
see all -
別名
- B cell receptor associated protein BAP37 antibody
- B-cell receptor-associated protein BAP37 antibody
- BAP antibody
see all
画像
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All lanes : Anti-REA antibody (ab71970) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab71970 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab71970 は 3 報の論文で使用されています。
- Toki S et al. The survival and proliferation of osteosarcoma cells are dependent on the mitochondrial BIG3-PHB2 complex formation. Cancer Sci 112:4208-4219 (2021). PubMed: 34363714
- Sircoulomb F et al. ZNF703 gene amplification at 8p12 specifies luminal B breast cancer. EMBO Mol Med 3:153-66 (2011). ICC/IF ; Human . PubMed: 21328542
- He B et al. A repressive role for prohibitin in estrogen signaling. Mol Endocrinol 22:344-60 (2008). WB, IP ; Mouse . PubMed: 17932104