The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
RCE1 (RCE1 homolog, prenyl protein peptidase) is a member of the metalloproteinase family. It functions in the maintenance and processing of CAAX-type prenylated proteins, proteolytically removing the C-terminal three residues of farnesylated and geranylated proteins.