製品の概要

  • 製品名Anti-RCC2 antibody
    RCC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RCC2
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 475 and the C-terminus (residue 522) of human RCC2. (NP_061185.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
    • 配列類似性Contains 7 RCC1 repeats.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA1470 antibody
      • Protein RCC2 antibody
      • RCC 2 antibody
      • RCC1 like protein TD 60 antibody
      • RCC1-like protein TD-60 antibody
      • RCC2 antibody
      • RCC2_HUMAN antibody
      • Regulator of chromosome condensation 2 antibody
      • TD60 antibody
      • Telophase disk protein of 60 kDa antibody
      see all

    Anti-RCC2 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 56 kDa
      Observed band size : 56 kDa

    Anti-RCC2 antibody (ab70788) 使用論文

    ab70788 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 5 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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    投稿 Oct 30 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (gastric)
    Loading amount 15 µg
    Specification gastric
    Gel Running Conditions Non-reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 May 19 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"