製品の概要

  • 製品名Anti-RCC2 antibody
    RCC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RCC2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide (Human) corresponding to to a region between residues 1 and 50 of human RCC2. (NP_061185.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use a concentration of 2 - 5 µg/ml.

ターゲット情報

  • 機能Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
  • 配列類似性Contains 7 RCC1 repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1470 antibody
    • Protein RCC2 antibody
    • RCC 2 antibody
    • RCC1 like protein TD 60 antibody
    • RCC1-like protein TD-60 antibody
    • RCC2 antibody
    • RCC2_HUMAN antibody
    • Regulator of chromosome condensation 2 antibody
    • TD60 antibody
    • Telophase disk protein of 60 kDa antibody
    see all

Anti-RCC2 antibody 画像

  • All lanes : Anti-RCC2 antibody (ab70787) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab70787 at 1 µg/ml for WB, and for IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-RCC2 antibody (ab70787) 使用論文

ab70787 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am pleased to provide the following testing protocol for ab70787 RCC2 antibody in IP. Preparation of Cell Lysate used for Immunoprecipitation Reagents needed: PBS (Phosphate Buffered Saline, pH 7.2) NaCl 8.0 g...

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